1. Product title: L-Carnitine
L - carnitine | (R) - 3 - carboxylic - 2 - hydroxy - N, N, N - trimethyl ammonium hydroxide - 1 - c | carney dean vitamin BT | | D - carnitine (-) - carnitine | | L (R) - 3 - hydroxy - 4 - (trimethyl amino) butyrate | l-carnitine | L - carnitine | L- carnitine internal salt
2. Product parameter table:
|Appearance||White Crystalline Powder||White Crystalline Powder|
|Identification||IR & NMR spectrum||IR & NMR spectrum|
|Specific Rotation||(-)9.5° ~ (-)11.0°||(-)10.4°|
|PH||3.0 ~ 4.5||3.5|
|Loss On Drying||0.5%max||0.10%|
|Residue on ignition||0.50% max||0.20%|
|Heavy Metals(As Pb)||10ppm max||Complies|
|Total Plate Count||1000cfu/gm||Complies|
L-carnitine, also known as L-carnitine or transliteral carnitine, is a kind of amino acid that promotes the conversion of fat into energy. Red meat is the main source of l-carnitine, which has no side effects on human body. Different types of diets already contain 5-100 mg of l-carnitine, but the average person can only get 50 mg per day from meals, and vegetarians get less. The main physiological function of l-carnitine is to promote the conversion of fat into energy. Taking l-carnitine can reduce body fat and weight without reducing water and muscle.
4. Preparation of l-carnitine:
L-carnitine is naturally found in a variety of meats and dairy products, so it can be extracted directly from beef and milk containing L-carnitine. It has been reported that 0.6g crystalline carnitine can be extracted from 450g beef extract and 100g lactose powder containing 2% L-carnitine can be extracted from 56kg milk. However, the extraction method costs a lot and is not economically reasonable.
Studies have shown that L-carnitine also exists in many microorganisms, and l-carnitine can be accumulated by deep culture or solid fermentation of yeast, Aspergillus, penicillium, rhizopus and other microorganisms. However, because of the complexity of strain screening, the current fermentation level is still relatively low. It was reported that the l-carnitine accumulated 0.4% with 2% DL-carnitine as raw material and fermented at 25℃ for 44h.
The patent of dl-carnitine synthesis was reported abroad in 1953, and it had been industrialized in 1960s. Domestic 1982 also has as gastric medicine production and application. Starting from DL-carnitine, l-carnitine was obtained by chemical resolution using camphor acid, N-acetyl-D-glutamic acid or ethylbenyl-L -(+) tartaric acid as resolution agent. However, the racemization of carnitine is difficult and can not be recovered.
Enzymatic conversion of
This is the most studied and most promising method. Chemical synthesis of DL-carnitine can be used, first acetylation into amides or nitriles, and then the use of microbial enzymes for selective hydrolysis and resolution. Such as Zhongshan qing, pseudomonas and other microbial amidase selective hydrolysis of DL-carnitine amide or carnitine nitrile, can be prepared optical purity of more than 99% of L-carnitine. In addition, l-carnitine can also be prepared by enzymatic transformation of β -dehydrocarnitine, enzymatic hydrolysis of trans-croton betaine and enzymatic hydroxylation of γ -butylbetaine.
At present, only Switzerland, Italy, Japan and other countries in the international production. Jiangsu Institute of Microbiology in China is also developing enzymatic transformation research.
3- chloro-2 - hydroxypropyl trimethylamine chloride was prepared from epichlorohydrin and trimethylamine quaternary ammonium salt, and then cyanated to 3- cyano-2 - hydroxypropyl trimethylamine chloride, and then hydrolyzed with concentrated hydrochloric acid to get carnitine hydrochloride. Or with γ -bromoacetyl acetate as raw material, first with sodium tetrahydroboronate reduction to γ -bromo-β -hydroxybutyrate ethyl ester, and then with trimethylamine to generate quaternary ammonium salt, namely trimethylammonium bromide -β -hydroxybutyrate ethyl ester, and finally with hydrochloric acid hydrolysis of carnitine hydrochloride. It can also be extracted directly from beef and milk containing L-carnitine.
5. Pictures of the product: