Metformin Hydrochloride Fall Blood Sugar White Crystalline Or Crystalline Powder
1. Product title:Metformin hydrochloride
2. Pictures of the product:
|Product Name||Metformin hydrochloride||Specification||99%|
|Appearance||White crystalline or crystalline powder||Main Effect||
Fall blood sugar
|Brand Name||Kan Ying||Store-method||Cool Dry Place|
|Shelf Life||2 Years When
3. Product Description:
Metformin hydrochloride, the main ingredient is metformin hydrochloride, this product can reduce the fasting and postprandial hyperglycemia of Ⅱ type diabetes patients, HBALC can decrease by 1%-2%, is one of the commonly used hypoglycemic drugs. Congestive heart failure, liver and renal insufficiency, diabetes mellitus complicated with ketoacidosis and acute infection
4. In vitro studies:
Metformin inhibited ESC proliferation in a concentration-dependent manner. The IC50 of A-ESC was 2.45mM and that of N-ESC was 7.87mM. Metformin significantly activates AMPK signaling in secretory A-ESC cells compared with proliferative cells . Metformin (0-500μM) reduced glycogen synthesis in a dose-dependent manner with IC50 values of 196.5μM in cultured rat hepatocytes . Metformin showed cell viability and cytotoxic effects on PC-3 cells with IC50 of 5 mM .
5. In vivo study:
Metformin alone (100 mg/kg, orally) and metformin with the isoproterenol group (25,50,100 mg/kg) were used to alleviate myocyte necrosis by histomathological analysis . Metformin (>; 900mg/kg/day, oral) resulted in near-death/death and signs of clinical toxicity in CRL: CD (SD) rats .
6. Cell experiment:
ESC was inoculated in 96-well plates at a concentration of 1×10 3 cells/well. After attachment, the cells were treated with different doses of metformin/compound C for 0 min, 15 min, 1 h and 24 h. MTT assay was performed as described earlier. In brief, MTT (5mg/mL) was added to the 96-well plate at 10μL/ well, and the plate was incubated for 4 h. The MTT reaction was terminated by removing the medium containing MTT, and 100μLDMSO was added to each well and incubated on an oscillator for 10 min at room temperature to ensure full crystal dissolution. The absorbance was measured at 595nm. Cell proliferation (percentage of control) was calculated as follows: absorbance (experimental group)/absorbance (control group). Cell proliferation inhibition (percent of control) was calculated as follows: 100% cell proliferation (percent of control). Each experiment was carried out in duplicate and repeated six times to assess consistency.
7. Experiments on animals:
The animals were randomly divided into 6 groups with 6 rats in each group. Rats in group 1 (control) received subcutaneous injection of normal saline (0.5mL) and were left untreated throughout the experiment. Rats in group 2 were given metformin orally (100mg/kg; Twice daily) for 2 days and subcutaneously injected with saline at 24 hour intervals for 2 consecutive days. Rats in group 3 (MI control) were given saline orally (twice a day) for 2 days, followed by subcutaneous injection of isoproterenol (100mg/kg) daily for 2 consecutive days, with an interval of 24 hours. Rats in groups 4 to 6 were treated with 25,50 and 100mg/kg of metformin. Metformin was dissolved in saline and force-fed at a volume of 0.25-0.5mL twice daily at 12-hour intervals, beginning immediately before isoproterenol injection.
8. Physical and chemical properties of metformin hydrochloride:
Boiling point 224.1 ° C at 760 mmHg
Melting point of 223-226 ° C (lit.)
Molecular formula C4H12ClN5
The molecular weight of 165.625
Flash point 89.3 DHS C
Accurate mass 165.078125
Appearance properties Crystalline solid
Vapor pressure 0.0929mmHg at 25°C
Store in a cool, dry place
The stability of
Stable under normal temperature and pressure, avoid contact with strong oxides
9. Pictures of the product: